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Real Time PCR LRTP-A40 a powerful, precise, modular design PCR thermal cycler system with 4-fluorescence detection channel. Based on advanced Peltier-based technology with high amplification efficiency up to 5°C/s maximum ramp rate saves valuable time. A powerful optical system offers Automatic collection of data from all wells during the detection process using white LED as a light source and a high-resolution CCD camera. Tailored with 470 nm, 525 nm, 585 nm, 610 nm Excitation filters, and 525 nm, 570 nm, 610 nm, 678 nm detection filters alongside different fluorescent channels like Channel 1: FAM/SYBR, Channel 2: VIC/HEX/JOE/TET/TAMRA/CY3, Channel 3: ROX/TexasRed, Channel 4: CY5, for various applications. Independent software analysis module enables easy experiment setup and delivers faster results. Ingenious design with the standard configuration of 96-well plate to carry out the quantitative study in gene expression, genetic variation, genotyping, etc. using small amounts of DNA or RNA as a nucleic acid sample. This device can be customized with an optional 5th channel based on customer requirements.
| Fluorescence Detection Channel | 4 |
| Reactions Per Run | 96 |
| Block Format | 96-well, 0.2 ml |
| Light Source | High brightness monochrome LED |
| Detector | Highly sensitive cold light CCD |
| Dynamic Detection Range | 100 to 1010 |
| Sensitivity | 1 copy |
| Reaction Volume | 15 to 100 µl |
| Excitation Source | White LED |
| Excitation Filters | Channel 1: 470 nm, Channel 2: 525 nm, Channel 3: 585 nm, Channel 4: 610 nm |
| Detection Filters | Channel 1: 525 nm, Channel 2: 570 nm, Channel 3: 610 nm, Channel 4: 678 nm |
| Fluorescent Dyes | Channel 1: FAM/SYBR, Channel 2: VIC/HEX/JOE/TET/TAMRA/CY3, Channel 3: ROX/TexasRed, Channel 4: CY5 |
| Block Material | Peltier |
| Temperature Range | 0°C to 99°C |
| Temperature Accuracy | ± 0.1 °C |
| Temperature Uniformity | ± 0.4 °C (10 sec after reaching 95°C), ± 0.2 °C (10 sec after reaching 95°C) |
| Maximum Ramp Rate | 5°C/sec |
| Gradient Range | 30°C to 99°C |
| Chemistry | All real time PCR based analysis, flexibility without passive reference dye. |
| PC Operation System | Windows XP/Vista/7/8/10 |
| PCR Machine Operation System | Linux |
| CPU | A8 |
| Network | LAN/Wi-Fi |
| Application Available | Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection |
| Dimension (W×D×H) | 592×440×280 mm |
| Weight | 15 kg |
Real Time PCR widely implemented in vitro method for multiplication/amplification /quantification of fluorescence dye tagged DNA or RNA nucleic acid. Real-time PCR thermal cyclers are used to conduct the quantitative study in gene expression, genetic variation, genotyping, protein expression, etc. using small amounts of DNA or RNA as a nucleic acid sample. PCR machine is in high demand across Genetic Engineering, Virology, Biotechnology, Medicine, Molecular Biology, Forensic Science, and Pharma Industry.
