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Real Time PCR LRTP-A40

Real Time PCR LRTP-A40 a powerful, precise, modular design PCR thermal cycler system with 4-fluorescence detection channel. Based on advanced Peltier-based technology with high amplification efficiency up to 5°C/s maximum ramp rate saves valuable time. A powerful optical system offers Automatic collection of data from all wells during the detection process using white LED as a light source and a high-resolution CCD camera. Tailored with 470 nm, 525 nm, 585 nm, 610 nm Excitation filters, and 525 nm, 570 nm, 610 nm, 678 nm detection filters alongside different fluorescent channels like Channel 1: FAM/SYBR, Channel 2: VIC/HEX/JOE/TET/TAMRA/CY3, Channel 3: ROX/TexasRed, Channel 4: CY5, for various applications. Independent software analysis module enables easy experiment setup and delivers faster results. Ingenious design with the standard configuration of 96-well plate to carry out the quantitative study in gene expression, genetic variation, genotyping, etc. using small amounts of DNA or RNA as a nucleic acid sample. This device can be customized with an optional 5th channel based on customer requirements.

Real Time PCR LRTP-A40

Fluorescence Detection Channel 4
Reactions Per Run 96
Block Format 96-well, 0.2 ml
Light Source High brightness monochrome LED
Detector Highly sensitive cold light CCD
Dynamic Detection Range 100 to 1010
Sensitivity 1 copy
Reaction Volume 15 to 100 µl
Excitation Source White LED
Excitation Filters Channel 1: 470 nm, Channel 2: 525 nm, Channel 3: 585 nm, Channel 4: 610 nm
Detection Filters Channel 1: 525 nm, Channel 2: 570 nm, Channel 3: 610 nm, Channel 4: 678 nm
Fluorescent Dyes Channel 1: FAM/SYBR, Channel 2: VIC/HEX/JOE/TET/TAMRA/CY3, Channel 3: ROX/TexasRed, Channel 4: CY5
Block Material Peltier
Temperature Range 0°C to 99°C
Temperature Accuracy ± 0.1 °C
Temperature Uniformity ± 0.4 °C (10 sec after reaching 95°C), ± 0.2 °C (10 sec after reaching 95°C)
Maximum Ramp Rate 5°C/sec
Gradient Range 30°C to 99°C
Chemistry All real time PCR based analysis, flexibility without passive reference dye.
PC Operation System Windows XP/Vista/7/8/10
PCR Machine Operation System Linux
CPU A8
Network LAN/Wi-Fi
Application Available Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection
Dimension (W×D×H) 592×440×280 mm
Weight 15 kg

  • 3D design with well pressure distribution and heat preservation
    Unique sliding track convenient for sample operation
    Four different fluorescent channels namely, Channel 1: FAM/SYBR, Channel 2: VIC/HEX/JOE/TET/TAMRA/CY3, Channel 3: ROX/TexasRed, Channel 4: CY5
    Advanced Peltier-based technology with high amplification efficiency up to 5°C/s maximum ramp rate
    Factor calibration for optical and thermal accuracy
    6 mm aluminum alloy, durable design main body
    Adjustable footing designed to maintain a steady balance
    Linux operation system, equipped with A8 CPU for better user experience
    Connection with PC through Wi-Fi or LAN
    Low noise operation, low energy consumption, long life span
    Complied with Good laboratory practice standards like 21 CFR part 11

Real Time PCR widely implemented in vitro method for multiplication/amplification /quantification of fluorescence dye tagged DNA or RNA nucleic acid. Real-time PCR thermal cyclers are used to conduct the quantitative study in gene expression, genetic variation, genotyping, protein expression, etc. using small amounts of DNA or RNA as a nucleic acid sample. PCR machine is in high demand across Genetic Engineering, Virology, Biotechnology, Medicine, Molecular Biology, Forensic Science, and Pharma Industry.

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